454 sequencing of pooled BAC clones on chromosome 3H of barley
Abstract Background Genome sequencing of barley has been delayed due to its large genome size (ca.5,000Mbp).Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones.Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped